Selaginella moellendorffii:
Several cDNA libraries were made by JGI from RNA isolated from mixed tissues, including roots, juvenile and adult plants, including
stobili (reproductive tissue, but not gametophytes). cDNA was directionally cloned into one of two vectors (pMVSPORT6 and pMCL-cDNA;
Libraries:
CAOP mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
CAOS mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
CAOT mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
CAOU mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
CAOW mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
CAOX mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
CAOY mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
CAOZ mixed tissues; whole plants with roots and reproductive tissue DOE Joint Genome Institute
SmGB Selaginella moellendorffii - Genbank External Data -
Vectors
CAOP pMCL200_cDNA
CAOS pMCL200_cDNA
CAOT pMCL200_cDNA
CAOU pMCL200_cDNA
CAOW pCMVSPORT6
CAOX pCMVSPORT6
CAOY pCMVSPORT6
CAOZ pCMVSPORT6
The cloning sites are SalI and NotI (polyA tail end) for both vectors.
The sequences of the two vectors are
pMVSPORT6 and pMCL-cDNA
Cluster Results:
338 (cr_338)
Description: Selaginella moellendorffii v2.1.1 [blastn] (w/ end pair merging)
Alignment Engine: blastn
Clustering Parameters: 96% identity, 150 bp overlap
339 (cr_339)
Description: Selaginella moellendorffii v2.2.1 [malign] (w/ end pair merging)
Alignment Engine: malign
Clustering Parameters: 96% identity, end-pairs always in same cluster